TyBlue
TyBlue Uses, Dosage, Side Effects, Mechanism of Action, How Long Does it Take to Work? TyBlue is a brand name. Its generic name is Trypan Blue.
Introduction
TyBlue is a type of dye that is used to stain certain tissues and cells. It is widely used in clinical laboratories and in research laboratories during cell culture experiments. It is used to observe the cell count, cell size, cell morphology, and other integral cellular properties.Uses
TyBlue is commonly used as a vital stain for cell counting in certain cell culture procedures and for the assessment of cellular viability. It is also used to improve the contrast between neutral stains and lipid-containing materials, or between blue and red stains. In addition, it can be used to assess the efficiency of transfection of cells.Mechanism of Action
When it is used to assess cell viability, TyBlue is able to pass through the cell membrane of viable cells but is excluded by dead cells. Thus, dead cells take up the dye and appear to be stained, while live cells remain unstained.How Long Does It Take to Work?
The TyBlue stain will usually take several minutes to work, depending on the cell type and concentration used.Absorption
TyBlue is not systemically absorbed and can safely be used with tissue and cells without risk of systemic absorption.Route of Elimination
TyBlue is eliminated via renal excretion, as it is not absorbable.Dosage
The dosage of TyBlue will depend on the study or procedure it is used for. Generally, it is applied topically to the cells in a dilute solution, usually in concentrations ranging from 0.01-0.2% (equivalent to 100-2000 µg/mL).Administration
TyBlue is typically applied to cells in culture dishes or wells using a pipette.Side Effect
In general, TyBlue is relatively safe for use and does not present much side effects.Toxicity
TyBlue is not known to be toxic when used at appropriate doses.Precaution
Since TyBlue can be toxic if swallowed, eye contact or skin contact should be avoided. Wearing gloves is recommended when handling the dye.Interaction
TyBlue does not interact with other drugs or medical conditions.Disease Interaction
TyBlue does not interact with medical conditions or diseases.Drug Interaction
TyBlue does not interact with any drugs.Food Interactions
TyBlue does not interact with any food items.Pregnancy Use
TyBlue is believed to be safe for use during pregnancy although more research is needed to be certain.Lactation Use
TyBlue is believed to be safe for use in breastfeeding women although more research is needed to be certain.Acute Overdose
In the event of an acute overdose, medical attention should be sought immediately.Contraindication
There is no contraindication for the use of TyBlue.Use Direction
TyBlue should be used according to the instructions provided in the product label.Storage Condition
TyBlue should be stored in a dry, dark, cool place and should not be exposed to direct sunlight.Volume of Distribution
The volume of distribution for TyBlue is not known.Half Life
The half life of TyBlue is not known.Clearance
The clearance of TyBlue is not known.Here you find in details version of TyBlue